We played with e coli. What we did was we set a sort of standard to follow and compare to with our tampering of the e coli. What we had done was segregated a positive pGLO, and a negative pGLO into four groups. 2 positive and 2 negative. the positive contained plasmid for the DNA to produce and the Negative didn't offer any plasmid for the DNA. What had happened after we added just one e coli bacterium, is they reproduced up to 17 of them selves in some. But this only occurred in the positives. but the negatives didn't have any thing for the bacteria to produce off of.
What came out the next day was a fair amount of bacteria in the positives and nothing in the negatives. in the positive where the amp was added the bacteria glow under the black light. the other one just was white.
we even added ingredients to the bacteria late, and it still reproduced. this lab helped me gain better understanding about how bacteria like e coli and reproduce.
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